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By Karl Maramorosch, Gordon H. Sato

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Cochran, B. , Huang, A. , and Stiles, C. D. (1985). Cell 43, 793-800. ADVANCES IN CELL CULTURE, VOL. 6 MICROVASCULAR ENDOTHELIAL CELLS: ISOLATION, IDENTIFICATION, AND CULTIVATION Mary E. Gerritsen Department of Physiology New York Medical College Valhalla, New York t William Carley* and Anthony J. Milici Departments of Cell Biology* and Surgeryt Yale University School of Medicine New Haven, Connecticut I. Introduction II. Isolation and Cultivation of Microvascular Endothelial Cells from Different Vascular Beds A.

Closely apposed cells with a polygonal morphology). If grown in FCS instead of PDS, contaminating smooth muscle cells and pericytes appear as elongated, dispersed, rapidly growing cells that tend to migrate apart from one another after dividing. , up to 2 0 - 2 2 passages) and maintain the endothelial features of angiotensin-converting enzyme (ACE), factor VIII antigen, P G I 2, and P G E 2 synthesis, and a nonadherent surface for platelets (Gerritsen and Cheli, 1983). However, should cells be contaminated with smooth muscle cells or pericytes, it has been our experience that the cell lines rapidly lose their endothelial characteristics (probably due to overgrowth with other cell types).

Biophys. Acta 605, 3 3 - 9 1 . McGarry, T. , and Lindquist, S. (1985). Cell 42, 903-911. McLachlan, A. D. (1978). J. Mol. Biol. 124, 297-304. McLachlan, A. , and Stewart, M. (1975). J. Mol. Biol. 162, 693-698. McLachlan, A. , and Stewart, M. (1982). J. Mol. Biol. 162, 693-698. 32 ELAINE FUCHS ET AL. Magin, T. , Jorcano, J. , and Franke, W. W. (1983). EMBO J. 2, 1387-1392. , and Fuchs, E. (1984). Cell 39, 491-498. , and Fuchs, E. (1985). Proc. Natl. Acad. Sci. SA. 8 2 , 1 6 0 9 1613. , Wakefield, L.

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